Review



reverttm wash solution  (LI-COR)


Bioz Verified Symbol LI-COR is a verified supplier
Bioz Manufacturer Symbol LI-COR manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    LI-COR reverttm wash solution
    Reverttm Wash Solution, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+solution/REVERT+700+WASH+SOLUTION/bio_rxiv__64898__2026__05__01__722223-593-5-8
    Average 96 stars, based on 54 article reviews
    reverttm wash solution - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Revert total protein normalization method offers a reliable loading control for mitochondrial samples following TBI.
    Article Snippet: After transfer, PVDF membranes were washed 3x with Tris-buffered saline (TBS, Fishers, 7447-40-7) and stained for total protein detection using the LI-COR REVERTTM kit, according to the manufacturer’s instructions. .. Membranes were submerged in REVERT 700 stain for 10 min, subsequently washed twice (2x) for 1 min each with wash solution (LI-COR, 6.7% (v/v) glacial acetic acid, 30% (v/v) methanol, in water), and scanned instantly at 700 nm channel on the Odyssey imaging system (LI-COR). .. The RTP stain was then removed by 15 min incubation in reversal solution (LI-COR, 0.1 M sodium hydroxide, 30% (v/v) methanol, in water) followed by 3x quick TBS washes.

    Article Title: Time Course of Mitochondrial Antioxidant Markers in a Preclinical Model of Severe Penetrating Traumatic Brain Injury
    Article Snippet: After transfer, PVDF membranes were stained for total protein detection using the REVERTTM kit (LI-COR) following the manufacturer’s instructions. .. Membranes were submerged in Revert stain for 10 min, washed twice (2×) for 1 min each with wash solution (LI-COR, 6.7% (v/v) glacial acetic acid, 30% (v/v) methanol, in water), and imaged immediately at 700 nm channel of the Odyssey imaging system (LI-COR). .. The total protein stain was then removed by 15 min incubation in reversal solution (LI-COR, 0.1 M sodium hydroxide, 30% (v/v) methanol, in water) followed by 3× quick Tris Buffer Saline (TBS, Fishers, 7447-40-7, Hampton, NH, USA) washes.

    Article Title: Temporal Changes in Mitochondria-Centric Excitotoxic Responses Following Severe Penetrating Traumatic Brain Injury.
    Article Snippet: After transfer, PVDF membranes were stained for total protein detection using the REVERTTM kit (LI-COR, Cat # 926-11010) following the manufacturer’s instructions. .. Membranes were submerged in Revert stain for 10 min, washed twice for 1 min each with wash solution (LI-COR, 6.7% (v/v) glacial acetic acid, 30% (v/v) methanol, in water), and imaged immediately at the 700 nm channel of the Odyssey imaging system (LI-COR). .. The total protein stain was then removed by 15 min incubation in reversal solution (LI-COR, 0.1 M sodium hydroxide and 30% (v/v) methanol in water) followed by three quick washes in Tris Buffer Saline (TBS, Fishers, Cat # 7447-40-7, Hampton, NH, USA).

    Article Title: Acute microtubule changes linked to DMD pathology are insufficient to impair contractile function or enhance contraction-induced injury in healthy muscle
    Article Snippet: .. Homogenized cell lysates were processed via SDS-PAGE (4-20% BioRad Mini-PROTEAN® TGXTM precast gels), transferred to a membrane (Millipore Immobilon-FL PVDF), stained for total protein (Revert 700 Total Protein Stain, P/N 926-11011, LI-COR Biotechnology) for 5 min at room temperature, then washed in Wash solution (P/N 926-11012, LI-COR Biotechnology) and finally in ultrapure water. .. Membranes were imaged on a LI-COR Odyssey CLx imager.

    Article Title: An autocrine synergistic desmin-SPARC network promotes cardiomyogenesis in cardiac stem cells.
    Article Snippet: .. Protein samples were mixed with 4× Protein Sample Loading Buffer (928–40,004, LiCOR), boiled at 95 ◦C for 5 min, centrifuged at 14,000 rpm at RT for 2 min and separated on precast 10 % SDS-PAGE gels (4,561,036, Bio-Rad) at 100 V for 70 min. Proteins were transferred to nitrocellulose blotting membranes (10–6000-01, VWR) in Harlow buffer at 350 mV and 4 ◦C for 2 h. The membrane was then washed in TBS and stained with RevertTM 700 Total Protein Stain and Wash Solution (926–11,015, LiCOR) and analyzed using an Odyssey CLx Fluorescence Imager (LiCOR). ..

    Article Title: A Synergistic Desmin-SPARC Axis Influences Cardiac Stem Cell Differentiation and Promotes Cardiomyogenesis through Autocrine Regulation
    Article Snippet: .. The membrane was then washed in TBS and stained with RevertTM 700 Total Protein Stain and Wash Solution (926-11015, LiCOR) and analyzed using an Odyssey CLx Fluorescence Imager (LiCOR). ..

    Imaging:

    Article Title: Revert total protein normalization method offers a reliable loading control for mitochondrial samples following TBI.
    Article Snippet: After transfer, PVDF membranes were washed 3x with Tris-buffered saline (TBS, Fishers, 7447-40-7) and stained for total protein detection using the LI-COR REVERTTM kit, according to the manufacturer’s instructions. .. Membranes were submerged in REVERT 700 stain for 10 min, subsequently washed twice (2x) for 1 min each with wash solution (LI-COR, 6.7% (v/v) glacial acetic acid, 30% (v/v) methanol, in water), and scanned instantly at 700 nm channel on the Odyssey imaging system (LI-COR). .. The RTP stain was then removed by 15 min incubation in reversal solution (LI-COR, 0.1 M sodium hydroxide, 30% (v/v) methanol, in water) followed by 3x quick TBS washes.

    Article Title: Time Course of Mitochondrial Antioxidant Markers in a Preclinical Model of Severe Penetrating Traumatic Brain Injury
    Article Snippet: After transfer, PVDF membranes were stained for total protein detection using the REVERTTM kit (LI-COR) following the manufacturer’s instructions. .. Membranes were submerged in Revert stain for 10 min, washed twice (2×) for 1 min each with wash solution (LI-COR, 6.7% (v/v) glacial acetic acid, 30% (v/v) methanol, in water), and imaged immediately at 700 nm channel of the Odyssey imaging system (LI-COR). .. The total protein stain was then removed by 15 min incubation in reversal solution (LI-COR, 0.1 M sodium hydroxide, 30% (v/v) methanol, in water) followed by 3× quick Tris Buffer Saline (TBS, Fishers, 7447-40-7, Hampton, NH, USA) washes.

    Article Title: Temporal Changes in Mitochondria-Centric Excitotoxic Responses Following Severe Penetrating Traumatic Brain Injury.
    Article Snippet: After transfer, PVDF membranes were stained for total protein detection using the REVERTTM kit (LI-COR, Cat # 926-11010) following the manufacturer’s instructions. .. Membranes were submerged in Revert stain for 10 min, washed twice for 1 min each with wash solution (LI-COR, 6.7% (v/v) glacial acetic acid, 30% (v/v) methanol, in water), and imaged immediately at the 700 nm channel of the Odyssey imaging system (LI-COR). .. The total protein stain was then removed by 15 min incubation in reversal solution (LI-COR, 0.1 M sodium hydroxide and 30% (v/v) methanol in water) followed by three quick washes in Tris Buffer Saline (TBS, Fishers, Cat # 7447-40-7, Hampton, NH, USA).

    SDS Page:

    Article Title: Acute microtubule changes linked to DMD pathology are insufficient to impair contractile function or enhance contraction-induced injury in healthy muscle
    Article Snippet: .. Homogenized cell lysates were processed via SDS-PAGE (4-20% BioRad Mini-PROTEAN® TGXTM precast gels), transferred to a membrane (Millipore Immobilon-FL PVDF), stained for total protein (Revert 700 Total Protein Stain, P/N 926-11011, LI-COR Biotechnology) for 5 min at room temperature, then washed in Wash solution (P/N 926-11012, LI-COR Biotechnology) and finally in ultrapure water. .. Membranes were imaged on a LI-COR Odyssey CLx imager.

    Article Title: An autocrine synergistic desmin-SPARC network promotes cardiomyogenesis in cardiac stem cells.
    Article Snippet: .. Protein samples were mixed with 4× Protein Sample Loading Buffer (928–40,004, LiCOR), boiled at 95 ◦C for 5 min, centrifuged at 14,000 rpm at RT for 2 min and separated on precast 10 % SDS-PAGE gels (4,561,036, Bio-Rad) at 100 V for 70 min. Proteins were transferred to nitrocellulose blotting membranes (10–6000-01, VWR) in Harlow buffer at 350 mV and 4 ◦C for 2 h. The membrane was then washed in TBS and stained with RevertTM 700 Total Protein Stain and Wash Solution (926–11,015, LiCOR) and analyzed using an Odyssey CLx Fluorescence Imager (LiCOR). ..

    Membrane:

    Article Title: Acute microtubule changes linked to DMD pathology are insufficient to impair contractile function or enhance contraction-induced injury in healthy muscle
    Article Snippet: .. Homogenized cell lysates were processed via SDS-PAGE (4-20% BioRad Mini-PROTEAN® TGXTM precast gels), transferred to a membrane (Millipore Immobilon-FL PVDF), stained for total protein (Revert 700 Total Protein Stain, P/N 926-11011, LI-COR Biotechnology) for 5 min at room temperature, then washed in Wash solution (P/N 926-11012, LI-COR Biotechnology) and finally in ultrapure water. .. Membranes were imaged on a LI-COR Odyssey CLx imager.

    Article Title: An autocrine synergistic desmin-SPARC network promotes cardiomyogenesis in cardiac stem cells.
    Article Snippet: .. Protein samples were mixed with 4× Protein Sample Loading Buffer (928–40,004, LiCOR), boiled at 95 ◦C for 5 min, centrifuged at 14,000 rpm at RT for 2 min and separated on precast 10 % SDS-PAGE gels (4,561,036, Bio-Rad) at 100 V for 70 min. Proteins were transferred to nitrocellulose blotting membranes (10–6000-01, VWR) in Harlow buffer at 350 mV and 4 ◦C for 2 h. The membrane was then washed in TBS and stained with RevertTM 700 Total Protein Stain and Wash Solution (926–11,015, LiCOR) and analyzed using an Odyssey CLx Fluorescence Imager (LiCOR). ..

    Article Title: A Synergistic Desmin-SPARC Axis Influences Cardiac Stem Cell Differentiation and Promotes Cardiomyogenesis through Autocrine Regulation
    Article Snippet: .. The membrane was then washed in TBS and stained with RevertTM 700 Total Protein Stain and Wash Solution (926-11015, LiCOR) and analyzed using an Odyssey CLx Fluorescence Imager (LiCOR). ..

    Fluorescence:

    Article Title: An autocrine synergistic desmin-SPARC network promotes cardiomyogenesis in cardiac stem cells.
    Article Snippet: .. Protein samples were mixed with 4× Protein Sample Loading Buffer (928–40,004, LiCOR), boiled at 95 ◦C for 5 min, centrifuged at 14,000 rpm at RT for 2 min and separated on precast 10 % SDS-PAGE gels (4,561,036, Bio-Rad) at 100 V for 70 min. Proteins were transferred to nitrocellulose blotting membranes (10–6000-01, VWR) in Harlow buffer at 350 mV and 4 ◦C for 2 h. The membrane was then washed in TBS and stained with RevertTM 700 Total Protein Stain and Wash Solution (926–11,015, LiCOR) and analyzed using an Odyssey CLx Fluorescence Imager (LiCOR). ..

    Article Title: A Synergistic Desmin-SPARC Axis Influences Cardiac Stem Cell Differentiation and Promotes Cardiomyogenesis through Autocrine Regulation
    Article Snippet: .. The membrane was then washed in TBS and stained with RevertTM 700 Total Protein Stain and Wash Solution (926-11015, LiCOR) and analyzed using an Odyssey CLx Fluorescence Imager (LiCOR). ..

    other:

    Article Title: Temporal Changes in Mitochondria-Centric Excitotoxic Responses Following Severe Penetrating Traumatic Brain Injury
    Article Snippet: Membranes were submerged in Revert stain for 10 min, washed twice for 1 min each with wash solution (LI-COR, 6.7% ( v / v ) glacial acetic acid, 30% ( v / v ) methanol, in water), and imaged immediately at the 700 nm channel of the Odyssey imaging system (LI-COR).

    Article Title: Pathological α-synuclein dysregulates epitranscriptomic writer METTL3 to drive neuroinflammation in microglia
    Article Snippet: For instances where total protein stain was applied, membranes were incubated in 520 Total Protein Stain (LI-COR) for 5 min followed by incubation with wash solution (LI-COR) for 5 min. Blots were scanned for normalization then destained by a 10-min incubation with destaining solution (LI-COR).



    Similar Products

    96
    LI-COR reverttm wash solution
    Reverttm Wash Solution, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+solution/REVERT+700+WASH+SOLUTION/bio_rxiv__64898__2026__05__01__722223-593-5-8
    Average 96 stars, based on 1 article reviews
    reverttm wash solution - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    LI-COR revert 700 total protein stain
    Revert 700 Total Protein Stain, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+solution/REVERT+700+TOTAL+PROTEIN+STAIN+%26+WASH+SOLUTION+KIT/pmc13628706-281-20-25
    Average 96 stars, based on 1 article reviews
    revert 700 total protein stain - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    94
    PolyAn GmbH polyan blocking solution
    Polyan Blocking Solution, supplied by PolyAn GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+solution/PolyAn+Blocking+Buffer+100+%2B+PolyAn+Washing+Buffer+Set+100/pm42329368-172-7-10
    Average 94 stars, based on 1 article reviews
    polyan blocking solution - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    94
    Miltenyi Biotec automacs pro washing solution
    Automacs Pro Washing Solution, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+solution/autoMACS+Washing+Solution/pmc13101284-58-0-5
    Average 94 stars, based on 1 article reviews
    automacs pro washing solution - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    94
    Miltenyi Biotec automacs washing solution
    Automacs Washing Solution, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+solution/autoMACS+Washing+Solution/pmc13264222-9-0-4
    Average 94 stars, based on 1 article reviews
    automacs washing solution - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    86
    Fisher Scientific eye wash buffer ewb solution
    Eye Wash Buffer Ewb Solution, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+solution/buffer+mcilvaine+na2edta+solution/us12648892-613-5-18
    Average 86 stars, based on 1 article reviews
    eye wash buffer ewb solution - by Bioz Stars, 2026-11
    86/100 stars
      Buy from Supplier

    96
    Valiant Co Ltd wash solution
    Wash Solution, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+solution/Tween+80/pm42045408-344-8-60
    Average 96 stars, based on 1 article reviews
    wash solution - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    97
    DeNovix sds washing solution
    Sds Washing Solution, supplied by DeNovix, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+solution/Microvolume+Spectrophotometer+And+Fluorometer+DeNovix+DS-11+FX/pm42066714-88-6-17
    Average 97 stars, based on 1 article reviews
    sds washing solution - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    97
    Thermo Fisher washing solution
    Washing Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+solution/Hanks'+Balanced+Salt+Solution+(1X)%2C+without+calcium%2C+without+magnesium%2C+without+phenol+red/pm41997233-65-7-13
    Average 97 stars, based on 1 article reviews
    washing solution - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    96
    LI-COR li cor odyssey instrument
    Li Cor Odyssey Instrument, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+solution/REVERT+700+TOTAL+PROTEIN+STAIN+%26+WASH+SOLUTION+KIT/pmc13105876-119-9-12
    Average 96 stars, based on 1 article reviews
    li cor odyssey instrument - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    Image Search Results